Project Overview
User-grade direct laser writing (DLW) enables the realization of three-dimensional (3D) objects, in relatively short time, at moderate costs.
However, spatial resolution is generally well above the diffraction limit. Instead, as shown in literature, 3D structures with submicron resolution can be obtained via multiphoton polymerization techniques (MPP), for which fabrication rates are significantly slower than DLW and equipment costs are orders of magnitude greater. In addition, most constructs for biomedical applications realized via DLW or MPP are based on oversimplifying geometries and generic models, and therefore do not accurately mimic patientspecific biological structures (e.g., tissues, blood vessels).
INTEGRAM aims at integrating the DLW and MPP additive manufacturing techniques in a single device, able to provide 3D polymeric constructs with high throughput and high resolution. Indeed, INTEGRAM will move beyond a merely technological purpose, by providing a robust workflow to fabricate patient-specific biomedical constructs, and validate their functional and therapeutic potential in vitro.
In particular, INTEGRAM will firstly set up a hybrid DLW/MPP fabrication device, based on sequential polymerization of low-resolution constructs, refined by high-resolution functional details. This will enable the realization of meso-/macroscale objects with (sub)micron features at unprecedented fabrication rates. Moreover, a library of photosensitive polymeric materials will be established.
To demonstrate the potential of the device, defective or damaged tissues (e.g., aortic stenosis) will be identified via segmentation algorithms starting from realistic diagnostic imaging, and a template for their reconstruction will be fabricated. Finally, biological functionality of the constructs will be validated in vitro.
Thus, INTEGRAM will pioneer the integration of precision additive manufacturing, personalized treatment, and cost sustainability of processes, in agreement with the three key technology impact pathways (i.e., scientific, societal and economic) set by Horizon Europe. Scientific outcomes of INTEGRAM will provide new basic and applied knowledge, boost the field of advanced manufacturing and play a pivotal role in future personalized, technology-driven healthcare.
Objectives
Objective 1
Implementation of multiscale Hi-R, Hi-T L-AM machinery
Objective 2
Development of personalized constructs
Objective 3
Realization of functional biomedical constructs
Objectives 4-6
Safety issues, Ethics and Regulatory acpects, Exploitation Strategy
